Cloning, Expression, Purification of PDI Gene from Wheat ‘Shaan 253’
Keywords Wheat, Protein Disulfide Isomerase (Pdi), Gene Cloning, Prokaryotic Expression, Protein Purification Abstract In order to further study the key protein that controls the formation of disulfide bonds in wheat proteins, namely protein disulfide isomerase (PDI), the cDNA sequence of the PDI gene of wheat variety “Shaan 253” was cloned using RTPCR method. The gene […]