Keywords

Common Wheat, Deoxyhaemophenol (Don), Abc Transporter, Pdr

Abstract

Deoxynivalenol (DON) plays an important role in the pathogenesis of wheat scab and is a toxic factor. The infection ability of Fusarium graminearum depends on its ability to produce DON, and disease-resistant varieties can Significantly reduce the content of DON in diseased panicle tissue. This study used Affymetrix wheat genome chips to analyze the gene expression characteristics of the panicle tissue after DON induction in the head blight-resistant wheat variety Wangshuibai. The results found that a protein encoding a PDR-type transporter The EST increased its expression by 45 times after being induced by DON. Based on the EST, primers were designed to screen the wheat genome TAC library and a TAC single clone containing the gene was obtained. The single clone was sequenced using chromosome walking, and gene prediction was performed using Softberry software. Primers were designed for the 5¡ä and 3¡ä untranslated regions of the gene, and the transporter gene was cloned from the DON-induced cDNA of wheat Wangshui white ear tissue. The full length of the genome is 7377bp, including 19 exons, and the CDS length is 4308bp. It encodes a protein with a length of 1435aa and a molecular weight of 161kD. Protein sequence comparison shows that the gene belongs to the PDR protein family and is named TaPDR1 (Triticumasetivum Pleiotropic DrugResistance). A set of Chinese spring deficiency-four systems was used to locate the TaPDR1 gene on the wheat chromosome 5A. Half Quantitative RT-PCR showed that the expression of TaPDR1 was induced by DON and Fusarium graminearum in Wangshuibaisui, indicating that it is involved in plant disease resistance defense responses. The gene was expressed at low levels in Wangshuibai susceptible mutants, further proving that TaPDR1 is related to Related to scab resistance. The expression of TaPDR1 is not induced by hormones related to biotic stress (JA and SA) and abiotic stress factors (heat, cold, injury and NaCl), but is induced by Al3+ and free Ca2+. It is speculated that [Ca2+]i mediates the expression signal of TaPDR1.

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Original research done by Shang Yi, Xiao Jin, Ma Lulin, Wang Haiyan, Qi Zengjun, Chen Peidu, Liu Dajun, Wang Xiu’e

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