Continuous Progressive Extraction Analysis Of Protein Components
Keywords
Protein Components, Rapid Separation, Detection
Abstract
In 1895, Osdorne first extracted and analyzed four protein components: albumin, globulin, prolamin and clutelin based on the characteristics of grain proteins that can be dissolved in different solvents. In 1962, based on previous research, Maes conducted a systematic screening study on the solvents, concentrations, extraction speeds and nitrogen determination methods required for extraction, and proposed a continuous progressive extraction method of protein components and the corresponding Kjeldahl method. The nitrogen method is widely used. Compared with the centrifugal method, this method has a faster extraction speed, a larger sample dosage range, fewer types of reagents, and higher accuracy. However, it requires a large amount of reagents, a long working cycle, and high labor intensity. Based on the existing equipment, this article applies the continuous progressive extraction principle of MAES to extract and separate various protein components, and then use an automatic nitrogen determiner to determine the nitrogen content.
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Original research done by Deng Miao, Wei Yimin
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