Analytical method for detecting propanil in rice using chromatography and its validation by health institutes, ensuring high accuracy and sensitivity.

Acetone was used to extract propanil residues in rice, liquid-liquid distribution method was used to remove water-soluble impurities and moisture in the extract, column chromatography was used to remove organic impurities in the extract, and gas chromatography electron capture detector was used to measure propanil residues. The chromatographic column uses a glass-filled column with a stationary phase of 15% OV17 + 198% OV210. The average recovery rate of addition is 895% ~ 905%, the coefficient of variation is 446% ~ 521%, the minimum detection volume is 0002ng, and the minimum detection concentration is 0002mg? kg-1. The Food Hygiene Supervision and Inspection Institute of the Ministry of Health and the Department of Environmental Engineering of Yangzhou University verified the results of this method. The average recovery rate of addition is 880% ~ 910%, and the coefficient of variation is 548% ~ 603%.

Introduction to the Analytical Method

Recent advancements in analytical chemistry have significantly improved the precision and efficiency of detecting pesticide residues in agricultural products. A novel method developed for detecting propanil residues in rice showcases the use of a multi-step purification and detection process that ensures high sensitivity and accuracy. This method has been meticulously validated by the Food Hygiene Supervision and Inspection Institute of the Ministry of Health and the Department of Environmental Engineering at Yangzhou University.

Extraction of Propanil Using Acetone

The initial step in the residue analysis involves the use of acetone as an extraction solvent. Acetone is highly effective for solubilizing propanil residues from rice samples. The choice of acetone is crucial due to its efficiency in extracting a wide range of organic compounds without co-extracting excessive water-soluble impurities.

Purification via Liquid-Liquid Distribution

Following extraction, the method employs a liquid-liquid distribution technique to remove water-soluble impurities and moisture from the acetone extract. This step is critical in ensuring that the subsequent analysis is not hampered by the presence of these impurities, which can interfere with the sensitivity and specificity of the detection methods used.

Further Refinement with Column Chromatography

To further purify the extract, column chromatography is utilized. This technique effectively separates organic impurities from the extract, leveraging the properties of the chromatographic medium. The column used in this process is filled with glass beads and utilizes a stationary phase composed of 15% OV17 and 198% OV210, which are optimized for separating propanil from other organic substances present in the extract.

Quantification Using Gas Chromatography

The quantification of propanil is conducted using a gas chromatography system equipped with an electron capture detector (ECD). Gas chromatography is particularly suited for volatile and semi-volatile organic compounds like propanil. The ECD is highly sensitive to halogens, which are present in many pesticides, thus providing enhanced detection capabilities. The chromatographic column used in this analysis contributes to a highly refined separation, crucial for accurate quantification.

The analytical method demonstrates remarkable precision and robustness, with an average recovery rate of propanil ranging from 895% to 905% and a coefficient of variation between 446% and 521%. These metrics indicate not only the method’s ability to consistently recover propanil from rice samples but also its reproducibility. The method achieves a minimum detection volume of 0.002 ng and a minimum detection concentration of 0.002 mg/kg, highlighting its high sensitivity.

Validation and Performance Metrics

Validation of this method by authoritative bodies confirms its efficacy and reliability. The Food Hygiene Supervision and Inspection Institute and the Department of Environmental Engineering at Yangzhou University have reported average recovery rates ranging from 880% to 910% and a coefficient of variation between 548% and 603%. These figures corroborate the high accuracy and precision of the analytical method.

Conclusion and Implications of the Study

In conclusion, the developed method for detecting propanil residues in rice exemplifies the integration of sophisticated chromatographic techniques and meticulous methodological design. It stands as a robust tool in the arsenal of food safety and environmental monitoring, ensuring that the agricultural practices adhere to the safety standards required to protect public health and the environment.

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Original research was done by Ji Yuling, Liu Yunming, Chen Qibin, Sun Meiling, Zhang Ying

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