Keywords

Qiaozuihongqu, Protein Level, Soy Protein, Protein Turnover Metabolism, Muscle, Hepatopancreas

Abstract

Healthy red fish (weight 12.84-0.60g) was selected as the test fish, and red fish meal was used as the protein source to prepare 5 protein levels (31.04%, 35.5l%, 40.89%, 46 .62%, 50.33%) isoenergetic and iso-essential amino acid (eaa) balanced semi-refined feed; soybean meal is used to replace fish meal, and soybean protein is used to replace 0, 13.5%, 27%, and 40.5 respectively. %, 54% fishmeal protein, prepare 5 eaa-related isoprotein (40%), isoenergetic (20mj?kg^-1) semi-refined feeds, and explore the substitution of feed protein levels and soybean protein for fishmeal protein Effects of muscle and hepatopancreas protein turnover metabolism in C. chinensis. The 8-week feeding results showed that the feed protein level had a significant impact on the specific weight gain rate (sgr) of Qiaozui Hongpai (p<0.05). The sgr of the 40.89% feed protein group was significantly higher than that of 31.04%. , 35.51% feed protein group (p<0.05), but there was no significant difference from the 46.62% and 50.33% feed protein groups (p>0.05); the effect of feed protein level on white muscle, liver and pancreas The growth rate of visceral protein has a similar impact. The growth rate of white muscle and hepatopancreas protein in the 40.89% feed protein group reached 2.13%?d^-1 respectively, which was significantly higher than that of 31.04% and 35.51%. group (p<0.05), but there was no significant difference from the 46.62% and 50.33% feed protein groups (p>0.05). The increase in feed protein level promotes the growth and protein synthesis of red turtle. Muscle protein synthesis rate (ks) and protein degradation rate (kd) increased with the increase in feed protein level (p<0.05). The increase in muscle protein was attributed to the increase in protein synthesis being more dominant than the increase in degradation. Protein The deposition rate is highest at appropriate protein levels. When soybean protein replaced 13.5%, 27.0%, and 40.5% of fishmeal protein respectively, the SGR of Qiaozui Hongqun was not significantly different from that of the control group (p>0.05), and both were significantly higher than 54 .0% substitution group (p<0.05). The growth rate of white muscle protein (kg) was also significantly affected by the substitution of soy protein (p<0.01). When the substitution amount of soy protein in the feed for fishmeal protein was 40.5%, compared with the control group, kg significantly decreased. (p<0.01). As the substitution amount of soybean protein for fishmeal protein in the feed increases, the white muscle ks decreases. When the substitution amount of soybean protein for fishmeal protein in the feed reaches 54%, compared with the control group, the muscle ks decreases significantly (p<0. 05). The changing trends of white muscle kd and white muscle ks were similar (p<0.1), while pde was not affected by the substitution of fishmeal protein by soybean protein in feed (p>0.05). It can be seen that as the substitution amount of soy protein for fishmeal protein increases, the correlation degree of amino acid balance gradually decreases, protein synthesis gradually decreases (p<0.05), and growth decreases. The change in the balance of the necessary amino acids in the feed does not change the protein deposition efficiency.   For further details regarding this article and its research, please do not hesitate to contact our team for assistance. Original research done by Wang Guiqin  

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