Effect Of Nacl Stress On Protein Content Of Wheat Seed Embryos During Germination Period
Keywords
Wheat, Seed Embryo, Two-Dimensional Electrophoresis
Abstract
Abstract In order to compare the expression of embryonic proteins in the early germination stage of wheat seeds under different concentrations of salt stress, “Jinmai-47” was used as the test material. Seeds treated with 0, 0.1 and 0.2 mol/L were selected, and 2-DE technology was used to study the changes in hypoblast protein content at three concentrations. PDQuest image analysis software was used to analyze the changes in protein content under salt stress. It was detected that there were 121 spots in normal seed embryos, 32 protein spots were down-regulated in abundance, and 21 protein spots were up-regulated under 0.1 mol/L NaCl stress; Under 0.2 mol/L NaCl stress, the abundance of 46 protein spots was detected to be down-regulated, and the abundance of 16 protein spots was up-regulated. Research results show that salt stress inhibits and activates some protein contents and expressions in embryos during wheat seed germination to varying degrees.
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Original research done by Duan Jiangyan, Niu Wenting, Liu Xiangbiao
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