Keywords

Calcium Ion-Dependent Protein Kinase,Leaf Rust Fungus,Wheat

Abstract

Based on the non-affinity interacting SSH library constructed earlier in our laboratory, CDPK2-EST was found to be highly expressed 8 hours after inoculation from wheat leaves infected by leaf rust fungus. CDPKs, calcium ion-dependent protein kinases, are important factors in undertaking changes in Ca2+ flow in plant cells in response to various biotic and abiotic stresses. In order to further study the expression characteristics of CDPK2 in the interaction between wheat and leaf rust fungi, RT-PCR and Western-Blotting techniques were used to analyze CDPK2 in different affinity combinations after wheat was infected by leaf rust fungi at the mRNA level and protein level. The expression profile was tested. The results showed that the wheat CDPK2 gene was induced by leaf rust infection. The incompatible combination showed up-regulated expression of the gene at both the mRNA and protein levels 4 hours after inoculation, while the expression at the transcription level dropped to the control level at 16 hours after inoculation. ; At the protein level, its expression reached the maximum at 16h after inoculation, and then returned to the control level. In the affinity combination, the gene was slightly expressed at the mRNA level 8 hours after inoculation. At the protein level, its expression was up-regulated at 8 hours and 16 hours after inoculation, but its expression was lower than the incompatibility combination, and then tended to the control level. This result shows that the wheat CDPK2 gene is involved in the interaction process between wheat and leaf rust fungi, and plays a certain role in improving wheat’s resistance to leaf rust in this process. This result lays the foundation for in-depth exploration of the role and mechanism of wheat CDPK2 gene in wheat resistance to leaf rust infection.

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Original research done by Cheng Ziyi, Wei Fengju, Yan Aihua, Zhang Yunwei, Wang Dongmei

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