Advanced analytical method for detecting patulin in rice and millet, optimizing extraction, purification, and ensuring food safety with high accuracy.

By optimizing the extraction solvent and its volume, ultrasonic time and adsorbent, a fast, simple and accurate analytical method for the determination of patulin in rice and millet was established. Acetonitrile was used as the extraction solvent, and the ethylenediamine-N-propylsilane (PSA) adsorbent was used for purification. The supernatant was analyzed after passing through the membrane, and the multiple reaction monitoring (MRM) mode was used in the electrospray ionization (ESI) mode. Determination. The results showed that patulin showed good linearity in the range of 0.1~100.0 ¦Ìg/L, and the correlation coefficient was not less than 0.999 0. The detection limit of patulin in rice is 0.03 ¦Ìg/kg, the lower limit of quantitation is 0.1 ¦Ìg/kg, the recovery rate is 88.3%~91.5%, and the relative standard deviation (RSD) is 2.3%~6.0%; The detection limit of mycin is 0.1 ¦Ìg/kg, the lower limit of quantification is 0.3 ¦Ìg/kg, the recovery rate is 92.7%~103.8%, and the RSD is 2.2%~6.9%, which meets the requirements of trace analysis. The collected rice and millet samples were analyzed using this method, and no patulin was detected.

Introduction to Patulin Detection in Food Products

The detection and quantification of patulin, a mycotoxin found in various food products, pose significant challenges due to its potential health risks. Recent advancements in analytical methodologies have led to the development of a rapid, straightforward, and precise approach for determining the presence of patulin in rice and millet. This paper outlines the optimization of various parameters, including the extraction solvent and volume, ultrasonic time, and adsorbent, culminating in an efficient method leveraging acetonitrile for extraction and ethylenediamine-N-propylsilane (PSA) for purification.

Optimization of Extraction and Purification Methods

The method’s efficiency hinges on the meticulous selection of acetonitrile as the extraction solvent, renowned for its efficacy in isolating patulin from complex matrices such as rice and millet. Following extraction, the purification process employs PSA, an adsorbent that significantly enhances the purity of the sample by removing interfering substances, thus ensuring the accuracy of subsequent analyses.

Analytical Procedure and Method Validation

Post-purification, the sample undergoes analysis through a membrane, with the supernatant being subjected to scrutiny under multiple reaction monitoring (MRM) mode within the electrospray ionization (ESI) spectrum. This sophisticated approach allows for the precise identification and quantification of patulin, boasting an impressive linear range of 0.1 to 100.0 μg/L and a correlation coefficient exceeding 0.999, indicative of the method’s reliability and reproducibility.

Sensitivity and Accuracy in Patulin Detection

The method demonstrates exceptional sensitivity and accuracy, with a detection limit for patulin in rice at 0.03 μg/kg and a quantification lower limit of 0.1 μg/kg. The recovery rates ranged from 88.3% to 91.5%, with a relative standard deviation (RSD) between 2.3% and 6.0%. Similarly, for millet, the detection limit was identified at 0.1 μg/kg, with a quantification lower limit of 0.3 μg/kg. The recovery rates in millet were slightly higher, ranging from 92.7% to 103.8%, and the RSD spanned from 2.2% to 6.9%, underscoring the method’s efficacy and robustness for trace analysis in both matrices.

Application and Efficacy of the Developed Method

The developed method was applied to analyze collected samples of rice and millet, yielding no detectable levels of patulin, thereby confirming the method’s capability in screening for this mycotoxin. This not only attests to the potential safety of the analyzed samples but also highlights the method’s practical applicability in ensuring food safety.

Conclusion and Implications for Food Safety

In conclusion, the establishment of this analytical method marks a significant stride towards enhancing food safety protocols. By optimizing critical parameters such as the extraction solvent, ultrasonic time, and adsorbent, this method offers a rapid, simple, and accurate solution for the determination of patulin in rice and millet. Its high sensitivity, precision, and recovery rates meet the stringent requirements of trace analysis, making it an invaluable tool in the ongoing efforts to safeguard public health against the risks posed by mycotoxins in food products.

For futher details of this article and research, feel free to contact our team for assistance.
Original research was done by Wang Fuhua, Shi Jieping, Zhao Yarong, Huang Jianxiang

About ETprotein:

ETprotein, a reputable plant protein vegan protein Chinese factory manufacturer and supplier, is renowned for producing, stocking, exporting, and delivering the highest quality organic bulk vegan protein and plant proteins. They include Organic rice protein, clear rice protein, pea protein, clear pea protein, oat protein, watermelon seed protein, pumpkin seed protein, sunflower seed protein, mung bean protein, peanut protein, various of plant peptides etc. Their offerings, characterized by a neutral taste, non-GMO, allergen-free attributes, cater to a diverse range of industries. They serve nutraceutical, pharmaceutical, cosmeceutical, veterinary, as well as food and beverage finished product distributors, traders, and manufacturers across Europe, USA, Canada, Australia, Thailand, Japan, Korea, Brazil, and Chile, among others.

ETprotein specialization includes exporting and delivering tailor-made protein powder and finished nutritional supplements. Their extensive product range covers sectors like Food and Beverage, Sports Nutrition, Weight Management, Dietary Supplements, Health and Wellness Products, and Infant Formula, ensuring comprehensive solutions to meet all your protein needs.

As a trusted company by leading global food and beverage brands and Fortune 500 companies, ETprotein reinforces China’s reputation in the global arena. For more information or to get a free sample of their protein products, please contact them and email sales(at)ETprotein.com today.