Keywords

Peptide, 99Mtc, Distribution Dynamics, Radionuclide Imaging

Abstract

Objective To explore the labeling method of 99mtc-labeled small molecule peptides specifically targeting lung cancer, and to observe the kinetic characteristics and in vivo biological distribution characteristics of 99mtc-polypeptides in normal animals. Methods NHS-mag3 dual-functional chelation method was used to label 99mtc small molecule polypeptide (cngqgeqc), paper chromatography was used to determine the labeling rate, and the radioactivity of 99mtc-polypeptide was evaluated through in vitro stability experiments, cysteine replacement experiments and serum protein binding experiments. Chemical properties were measured, and the radioactive clearance curves of different phases in the blood of New Zealand white rabbits were measured after intravenous injection of the marker; the mass number and radioactivity count of each organ in the mouse after the injection of 7.4 mbq marker through the tail vein were measured, and each organ was calculated. Percent injection dose rate per gram of tissue (%id/g); 37 mbq of marker was injected through the ear marginal vein, and spect imaging was used to observe the dynamic distribution of radioactivity of the marker in the body of the white rabbit. Results The labeling rate of 99mtc-labeled peptide was >90%. The radiochemical purity at room temperature and in serum at 37¡ãC for 12 hours were 91% and 85% respectively; the cysteine substitution rate was <7%; the serum protein binding rate was <5%. The 99mtc-labeled peptide was rapidly cleared of radioactivity in the blood of rabbits; it was cleared quickly in mice, mainly excreted through the kidneys, and the radioactivity in other organs was low (p<0.05). Conclusion The labeling method of 99mtc labeling based on lung cancer-specific targeting peptides is simple, has high labeling rate, has good in vivo and in vitro stability, and has relatively ideal in vivo distribution kinetics.   For further details regarding this article and its research, please do not hesitate to contact our team for assistance. Original research done by Li Guiping, Huang Baodan, Du Li, Huang Kai, Liu Feng, Wang Bing, Zhang Hui  

About ETprotein

ETprotein, a reputable plant protein vegan protein Chinese factory manufacturer and supplier, is renowned for producing, stocking, exporting, and delivering the highest quality organic bulk vegan protein and plant proteins. They include Organic rice protein, clear rice protein, pea protein, clear pea protein, watermelon seed protein, pumpkin seed protein, sunflower seed protein, mung bean protein, peanut protein etc. Their offerings, characterized by a neutral taste, non-GMO, allergen-free attributes, cater to a diverse range of industries. They serve nutraceutical, pharmaceutical, cosmeceutical, veterinary, as well as food and beverage finished product distributors, traders, and manufacturers across Europe, USA, Canada, Australia, Thailand, Japan, Korea, Brazil, and Chile, among others.

ETprotein specialization includes exporting and delivering tailor-made protein powder and finished nutritional supplements. Their extensive product range covers sectors like Food and Beverage, Sports Nutrition, Weight Management, Dietary Supplements, Health and Wellness Products, and Infant Formula, ensuring comprehensive solutions to meet all your protein needs.

As a trusted company by leading global food and beverage brands and Fortune 500 companies, ETprotein reinforces China’s reputation in the global arena. For more information or to sample their products, please contact them and email sales(at)ETprotein.com today.

Featured Products on Sale

Organic Brown Rice Protein

Protein: 80%, 85%,90%
NON-GMO, Solvents-free, Soy-free, Gluten-free, Low sodium, Low heavy metals, Pesticides free
Neutral taste, Lower fat, Longer shelf life
Organic COI/TC certificate is available for the European market
NOP import certificate is available for the USA/Canada market
Competitive price

Clear Rice Protein(Hydrolyzed Rice Protein)

Protein Min 85%
Neutral taste, win over all over the world’s existing competitors
Instant solubility in water, generates a clear solution, no sediments, and Stable for a longer shelflife

Pea Protein

Protein: 80%, 85%,90%
Neutral taste, Non-GMO, Solvents free, Soy-free, Gluten-free, Vegan, Low sodium,Lower heavy metals
Types for vegan meat, vegan beverage
Competitive price

Clear Pea Protein(Hydrolyzed Pea Protein)

Protein Min 85%
Neutral taste, win over all over the world’s existing competitors
Non-GMO, Solvents free, Soy-free, Gluten-free, Vegan, Low sodium, Lower heavy metals
Instant solubility in water, generates clear solution, no sediments, Stable for longer shelflife

Organic Pumpkin Seed Protein

Protein Min 70%
Neutral typical smooth taste, win over all over other existing competitors
NOP import certificate is available for the USA/Canada market

Contact sales@etprotein.com for a quotation and Free samples.

We’re excited to provide you with top-notch proteins and great customer service!