Optimization Of The Process Of Preparing Rice Antioxidant Peptide Microcapsules By Orifice Method Using Response Surface Methodology
Exploring microencapsulation’s efficacy in enhancing rice antioxidant peptide stability using sodium alginate, optimized conditions, and significant results from storage and structural analysis.
In order to improve the stability of rice antioxidant peptides, sodium alginate was used as the wall material and the orifice method was used to microencapsulate the rice antioxidant peptides. Taking the embedding rate as the inspection index, based on the single factor experiment, the response surface analysis method was further used to analyze the mass ratio of the core material to the wall material, the mass fraction of sodium alginate, the embedding temperature, the mass fraction of CaCl2, and the mass fraction of sucrose fatty acid esters. The process conditions were analyzed and optimized, and the prepared microcapsules were subjected to storage experiments and electron microscope scanning. The results of the response surface optimization test show that the optimal process conditions for preparing rice antioxidant peptide microcapsules are the mass ratio of core material to wall material 0.3:1, sodium alginate mass fraction 1.4%, embedding temperature 50¡ãC, and CaCl2 mass fraction 1.8% , the mass fraction of sucrose fatty acid ester is 0.26%. It has been verified that the embedding rate of microcapsules prepared under the above optimal conditions is 81.24%. Compared with the model prediction value of 81.75%, the relative error is only 0.6%. The storage experiment results showed that the stability of the antioxidant peptides in the microcapsules was significantly improved; the scanning electron microscope results showed that the surface morphology and structure of the microcapsules were intact. Experimental results show that microencapsulation is a better method to protect the activity of antioxidant peptides, and can provide a reference for the actual production of rice antioxidant peptide microcapsules.
Introduction to Microencapsulation for Peptide Stability
The stabilization of bioactive peptides is pivotal in ensuring their efficacy and shelf-life, particularly when applied in the pharmaceutical and food industries. Rice antioxidant peptides, known for their beneficial health effects, are no exception. A recent study has demonstrated a novel approach to improving the stability of these peptides through microencapsulation, using sodium alginate as the wall material and employing the orifice method for the encapsulation process.
Microencapsulation: A Promising Protective Technique
Microencapsulation, a process that involves encasing tiny particles or droplets of a substance within a protective coating, has emerged as a promising technique to shield bioactive compounds from environmental factors that could degrade their efficacy. In this study, the effectiveness of microencapsulation in preserving the stability of rice antioxidant peptides was thoroughly investigated, with a keen focus on optimizing the encapsulation conditions to achieve the highest embedding rate – a critical measure of the efficiency of the encapsulation process.
Methodology and Optimization of Encapsulation Conditions
The research methodology hinged on a meticulous experimental design, starting with a single-factor experiment to establish a baseline understanding of the impact of various parameters on the embedding rate. Subsequently, the study leveraged response surface analysis, a statistical method used to model and optimize processes, to scrutinize and fine-tune five key factors: the mass ratio of core material to wall material, the mass fraction of sodium alginate, the embedding temperature, the mass fraction of CaCl2 (calcium chloride), and the mass fraction of sucrose fatty acid esters.
Optimal Conditions and Embedding Rate Achievement
The optimization trials revealed that the ideal conditions for preparing rice antioxidant peptide microcapsules were a core-to-wall material mass ratio of 0.3:1, a sodium alginate mass fraction of 1.4%, an embedding temperature of 50°C, a CaCl2 mass fraction of 1.8%, and a sucrose fatty acid ester mass fraction of 0.26%. Under these conditions, the embedding rate reached 81.24%, closely aligning with the model’s predictive value of 81.75% and demonstrating a negligible relative error of 0.6%.
Validation of Microencapsulation Effectiveness
To validate the effectiveness of the microencapsulation process, the study conducted storage experiments and utilized scanning electron microscopy to assess the microcapsules’ physical integrity over time. The results were unequivocal, showing a significant enhancement in the stability of the encapsulated antioxidant peptides, with the microcapsules maintaining their structural integrity throughout the storage period.
Conclusion: Implications for Rice Antioxidant Peptide Application
These findings underscore the potential of microencapsulation, particularly using sodium alginate as the wall material, as an efficient strategy to protect and stabilize bioactive peptides like those derived from rice. The optimized process conditions identified in this study not only pave the way for enhancing the shelf-life and efficacy of rice antioxidant peptides but also offer valuable insights that could be applied to the broader field of bioactive compound stabilization. This advancement holds promising implications for the production and application of rice antioxidant peptide microcapsules in various industries, offering a robust reference for future research and development endeavors in this arena.
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Original research was done by Zhang Yu, Lin Qinlu, Tan Yicheng, Chen Li
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