Discover the weight management benefits of green bean protein. Mitigate high-fat diet effects naturally. Boost metabolism and curb fat accumulation.

A recent animal study has revealed that green bean isolate protein can reduce diet-induced weight gain, fat accumulation, and liver fat degeneration (primarily leading to fatty liver) resulting from a high-fat diet.

Published in the Journal of Biochemistry and Biophysics Communications, researchers assigned regular-fed mice to either a high-fat diet or a high-fat diet supplemented with green bean isolate protein, spanning a total of 4 weeks. Simultaneously, germ-free mice were randomly allocated to either a high-fat diet or a high-fat diet enriched with green bean protein, also lasting for 4 weeks. Throughout the study, researchers measured body weight weekly and collected fecal samples for analysis. Subsequently, the mice were euthanized, and their liver, cecum, epididymis, perirenal fat tissue, and subcutaneous fat tissue were collected and weighed. Mice from regular and germ-free groups were examined to analyze and compare changes in gut microbiota.

The results indicated that mice on a high-fat diet enriched with green bean protein exhibited less weight gain by the end of the second week compared to those that did not receive the protein. The weight of epididymis, perirenal fat tissue, and subcutaneous fat tissue in the green bean protein group was also lower, although cecum weight did not show significant differences among the groups. Additionally, mice in the green bean protein group had lower liver weight, significantly reduced triglyceride levels in the liver, and decreased cytoplasmic vacuolization, indicating a mitigation of fat degeneration.

As mentioned earlier, germ-free mice were utilized to assess the impact of gut microbiota on beneficial metabolic effects. Results demonstrated a notable influence of gut microbiota in germ-free mice, as there were no significant differences in body and tissue weights or alleviation of liver fat degeneration between the high-fat diet group and the green bean protein-enriched high-fat diet group.

Mice fed with green bean protein also exhibited elevated levels of intestinal-mediated glucagon-like peptide-1 (GLP-1), indicating an increase in cecal bile acid content and secondary bile acid levels. Conversely, this effect was not observed in germ-free mice, suggesting that the beneficial effects of green bean isolate protein depend on the presence of gut microbiota.

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