Keywords

Chloroplast Protein, Subcellular Localization Methods

Abstract

Chloroplasts are important sites for photosynthesis in plants. Chloroplast proteins are distributed in different regions of chloroplasts according to different functions. Only when proteins are transported to specific locations can they perform their functions and participate in the life activities of cells. Therefore, determining the location of a protein in a cell is important for understanding its function and structure. In recent years, in the study of plant protein localization, bioinformatics prediction methods are commonly used to assist experimental methods to increase the accuracy of protein localization research. This article reviews the main advances in prediction methods and experimental methods commonly used in chloroplast protein localization research in recent years, providing a reference for efficient subcellular localization methods of chloroplast proteins. Chloroplast plays an important role in photosynthesis. Chloroplast proteins distribute in different structures in chloroplast according to their various functions. Proteins could be effective and take part in cell activities only after they are transported to special loci. Therefore, it is necessary to know the subcellular localization of chloroplast protein to understand the relationship between chloroplast structure and function. Recently, in the research of plant protein localization, some experimental methods assisted by bioinformatic prediction were used to increase the accuracy of protein localization analysis. Basic theory and main steps of these two methods were summarized in this paper in order to provide an effective method for chloroplast protein subcellular localization research.

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Original research done by Wang Yan, Na Jie

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