Advanced HPLC method for rapid, accurate detection of neonicotinoid pesticides in rice, showcasing high precision, sensitivity, and efficiency in food safety analysis.

method for the simultaneous determination of dinotefuran, clothianidin, imidacloprid, and imidacloprid in rice by high-performance liquid chromatography using a high-throughput (RRHT) chromatographic column was established. The sample was extracted with acetonitrile, extracted and purified using ENVI-Florisil and Carb composite solid-phase extraction columns, liquid chromatography high-throughput RRHT chromatographic column, 258nm ultraviolet measurement, and external standard method for quantification. The detection process is fast and accurate, and the four pesticides can be completely separated in 2.5 minutes. The spiked recovery rate in the sample was 75.5% to 96.0%, the relative standard deviation was 0.49% to 4.36%, and the lowest detection limit reached 0.004mg/kg. There is a good linear relationship between the peak area and the standard solution mass concentration in the range of 0.1 ~ 1.0 mg/L, and the linear regression coefficient is greater than 0.9996

Introduction to Pesticide Detection in Agriculture

In the ever-evolving landscape of agricultural science, ensuring the safety and quality of crops is paramount. One of the major concerns in this arena is the presence of pesticide residues in food crops such as rice, which is a staple diet for a significant portion of the global population. Addressing this concern, a novel method has been developed for the simultaneous determination of four neonicotinoid pesticides – dinotefuran, clothianidin, imidacloprid, and thiamethoxam – in rice utilizing high-performance liquid chromatography (HPLC). This cutting-edge technique employs a high-throughput Rapid Resolution High Temperature (RRHT) chromatographic column, marking a significant advancement in the field of agricultural chemistry and food safety analysis.

Advanced Methodology for Neonicotinoid Analysis

The methodological approach outlined for the analysis is both innovative and efficient. Initially, the rice sample is subjected to extraction using acetonitrile, a solvent well-regarded for its effectiveness in isolating pesticide residues from complex matrices. Following extraction, the sample undergoes purification through a dual-phase process utilizing ENVI-Florisil and Carb composite solid-phase extraction columns. This step is crucial for eliminating potential interferents, ensuring the purity of the sample for subsequent analysis.

Extraction and Purification Techniques

The chromatographic analysis is conducted using a state-of-the-art RRHT column, optimized for high-throughput analyses. Detection of the neonicotinoid pesticides is achieved at 258nm using ultraviolet measurement, a technique known for its sensitivity and specificity in detecting compounds of interest. Quantification of the pesticide residues is performed using the external standard method, a robust and reliable approach in analytical chemistry.

Rapid and Accurate Chromatographic Analysis

One of the most noteworthy aspects of this method is its rapidity and accuracy. The entire chromatographic separation and detection of the four pesticides can be accomplished within a mere 2.5 minutes, a remarkable achievement that significantly reduces analysis time without compromising the quality of results. The method demonstrates excellent analytical performance, with a spiked recovery rate ranging from 75.5% to 96.0%, and a relative standard deviation between 0.49% and 4.36%, indicating high precision and accuracy of the technique. Furthermore, the method achieves an exceptionally low detection limit of 0.004mg/kg, showcasing its sensitivity in detecting minute quantities of pesticide residues.

Exceptional Analytical Performance

Moreover, the technique exhibits a strong linear relationship between the peak area and the mass concentration of the standard solution within the range of 0.1 to 1.0 mg/L, with a linear regression coefficient greater than 0.9996. This indicates the method’s capability to provide reliable and consistent quantitative results across a relevant concentration range, making it an invaluable tool for monitoring the safety of rice and potentially other food crops.

Conclusion: Enhancing Food Safety with Innovative Technology

In conclusion, this novel HPLC method, with its high throughput, rapid analysis time, and excellent analytical performance, represents a significant advancement in the field of food safety and agricultural chemistry. It provides a powerful and efficient tool for the determination of neonicotinoid pesticide residues in rice, thereby contributing to the ongoing efforts to ensure the safety and quality of food products. This method not only underscores the importance of innovative analytical techniques in addressing food safety concerns but also sets a new benchmark for future developments in the field.

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Original research was done by Ding Ping, Yi Xiaojuan, Xia Yongjun, Zhu Jiaye

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