Keywords

Spirulina, Selenium, Biotransformation, Antioxidants, Free Radicals

Abstract

Abstract Purpose: To comparatively study and evaluate the in vitro free radical scavenging activity of the main selenium-rich active forms produced by biotransformation of selenium-rich spirulina (SeSP). Methods: SeSP was prepared using selenium-enriched culture technology, and selenium total protein (SeSPª²TP), selenium phycobiliprotein complex (SeSPª²PB), selenium phycocyanin (SeSPª²PC), and selenium were fractionated and purified from SeSP. Selenium polysaccharide ( SeSPª²PS) and nano red selenium (Nanoª²Se); inductively coupled plasma mass spectrometer (ICPª²MS) to determine selenium content; superoxide anion (¡¤O2) and hydroxyl radical (¡¤OH) chemiluminescence system to detect free radical scavenging rate, Reed-Muench algorithm calculates the half effective concentration (EC50). Results: The scavenging effects of SeSPª²TP, SeSPª²PB and SeSPª²PC on free radicals were significantly improved in the same form without selenium (P£¼0ª±05), and the EC on scavenging effects of ¡¤O2 and ¡¤OH was significantly improved. 50 significantly reduced (P <0ª±05), SeSPª²PB among SeSP has the highest free radical scavenging activity in vitro, among which SeSPª²PC may play a major role; the mass fraction of selenium in SeSPª²PS is low, and the enhancing effect of selenium on free radical scavenging is not yet known. It is clear; NanoSe transformed from microalgae also has strong free radical scavenging activity. Conclusion: The enhanced effect on free radical scavenging of the selenium-rich active form biotransformed by SeSP is related to the incorporation of active selenium elements. The selenium-rich protein converted by algae has an obvious synergistic effect. Biotransformation of nano-selenium as a new type of active Selenium speciation deserves further study.   For further details regarding this article and its research, please do not hesitate to contact our team for assistance. Original research done by Huang Feng, Guo Yunfei, Chen Yu, Ling Qinjie, Huang Zhi  

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